Mouse DNA Repair Protein RAD50 ELISA Kit from Innovative Research is intended for the quantitative determination of Mouse DNA Repair Protein RAD50 in biofluid samples, such as tissue homogenates, cell lysates and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . RAD50 present in samples reacts with an anti-Mouse RAD50 capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-RAD50 detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active RAD50 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Mouse RAD50. Detection Target: Mouse DNA Repair Protein RAD50 Reactivity: Mouse Range: 0.312-20ng/mL Sensitivity: 0.113ng/mL Storage Conditions: Store all components at 4┬░C This Mouse RAD50 activity assay is for the quantitative determination of DNA Repair Protein RAD50 in samples including tissue homogenates, cell lysates and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.